WET HEAT EXPOSURE: A POTENTIALLY REVERSIBLE CAUSE OF LOW SEMEN QUALITY IN INFERTILE MEN
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SHAI SHEFI, PHIROZ E. TARAPORE, THOMAS J. WALSH, MARY CROUGHAN, PAUL J. TUREK

Departments of Urology (SS, PET, TJW, PJT), Obstetrics, Gynecology and Reproductive Science (MC, PJT), and Epidemiology and Biostatistics (MC), University of California San Francisco, San Francisco, California, USA

ABSTRACT

     Objective: To evaluate the recovery of semen quality in a cohort of infertile men after known hyperthermic exposure to hot tubs, hot baths or whirlpool baths.
     Materials and Methods: A consecutive cohort of infertile men had a history remarkable for wet heat exposure in the forms of hot tubs, Jacuzzi or hot baths. Clinical characteristics and exposure parameters were assessed before exposure was discontinued, and semen parameters analyzed before and after discontinuation of hyperthermic exposure. A significant seminal response to withdrawal of hyperthermia was defined as ≥ 200% increase in the total motile sperm count (TMC = volume x concentration x motile fraction) during follow-up after cessation of wet heat exposure.
     Results: Eleven infertile men (mean age 36.5 years, range 31-44) exposed to hyperthermia were evaluated pre and post-exposure. Five patients (45%) responded favorably to cessation of heat exposure and had a mean increase in total motile sperm counts of 491%. This increase was largely the result of a statistically significant increase in sperm motility from a mean of 12% at baseline to 34% post-intervention (p = 0.02). Among non-responders, a smoking history revealed a mean of 5.6 pack-years, compared to 0.11 pack-years among responders. The prevalence of varicoceles was similar in both cohorts.
     Conclusions: The toxic effect of hyperthermia on semen quality may be reversible in some infertile men. We observed that the seminal response to exposure elimination varies biologically among individuals and can be profound in magnitude. Among non-responders, other risk factors that could explain a lack of response to elimination of hyperthermia should be considered.

Key words: male infertility; induced hyperthermia; semen; analysis; spermatogenesis
Int Braz J Urol. 2007; 33: 50-7

INTRODUCTION

     Spermatogenesis is sensitive to a variety of chemical and physical stressors. Testicular hyperthermia has been known to have a deleterious effect on male fertility since the time of Hippocrates and is a well-recognized cause of impaired sperm production (1). Its detrimental effect has been demonstrated in both animal models and in humans (2-4). Whether due to endogenous (such as high fevers) or exogenous stimuli, heat decreases sperm concentration, impairs motility, and reduces the number of morphologically normal sperm (5-8). This effect is striking enough that the effect of laptop computers on scrotal hyperthermia has recently been reported and the use of heat exposure as a male contraceptive has been studied (9,10). In contrast to the well described detrimental effects of dry heat on spermatogenesis, the consequences of wet heat exposure are relatively undefined. Our working hypothesis is that the effects of wet heat on spermatogenesis are similar to that from dry heat.
     The only published study to examine the effects of wet heat exposure on human fertility was performed by Rock and Robinson in 1965. In this study, the authors exposed 20 oligospermic men to wet heat (43-45°C) with a bottle warmer held between the thighs for 30 minutes on 6 alternating days. They noted a decline in sperm production that was followed by improvement in seminal parameters with cessation of the exposure, however the details of semen quality before and after exposure were not provided by the authors (11). The effects of more common exposures to wet heat, such as with hot tubs, Jacuzzis, or hot baths have not been addressed in the literature.
     The data provided by Rock and Robinson, as well as the well-defined association between dry heat exposure and impaired spermatogenesis led us to study the effect of total-body wet heat exposure in human males. We asked whether withdrawal of exposure in men with poor semen quality and a history of wet heat exposure could lead to an improvement in semen quality. We retrospectively compared semen quality before and after cessation of wet heat exposure, and also performed an interval analysis of responders and non-responders, looking for factors that might modulate responsiveness to therapeutic intervention.

MATERIALS AND METHODS

     Over a 3-year period, infertile men with exposure to wet heat were identified in a single, university-based, male infertility practice. Inclusion criteria were wet heat exposure, defined as the immersion of the body in a hot tub, heated Jacuzzi, or bath at a temperature warmer than body temperature, for ≥ 30 minutes per week during ≥ 3 months prior to presentation. All female partners were concurrently assessed for reproductive issues. Patients were excluded if they had received any medical or surgical infertility treatment within 1 year of study intake, and if a diagnosis of co-existing female factor infertility was made. Because patient data was decoded of all protected health information, a waiver was obtained from the Committee on Human Research for this study.
     All subjects underwent a complete history and physical examination by a single specialist (PJT). Information regarding the type, frequency, duration/episode and overall length of exposure to wet heat was collected during this visit. Counseling regarding the cessation of wet heat exposure was given during the initial visit. We attempted to obtain 2 semen samples for analysis at baseline (during exposure), < 3 months after intervention, and between 3-6 months after intervention in all patients. Semen was collected by masturbation after 2 to 3 days of abstinence and processed within 1 hour of ejaculation. All semen analyses were performed in a single andrology laboratory by the same technician, and assessed according to World Health Organization guidelines (12). Motility was reported as the sum of A + B patterns (12). A positive response to discontinuation of heat exposure was defined as an increase in total motile count (TMC = ejaculate volume x sperm concentration x fraction of motile sperm) > 200% during follow-up. This cutoff was selected to be above the natural variability in semen parameters within an individual: sperm volume, concentration and motility have been shown to vary by 59%, 54% and 96%, respectively, in a single individual with time (13). Statistical analysis was performed using two-tailed, paired “t” test to compare pre- and post-intervention seminal parameters. Probability values < 0.05 were considered significant.

RESULTS

Patient Characteristics
     Table-1 outlines the demographic and clinical characteristics of the 11 eligible study subjects. Mean patient age was 36.5 years, and mean partner age was 34.6 years. Couples had been trying to conceive for an average of 2.6 years (range 0 - 15 years) prior to evaluation. Nine patients (82%) had an evidence of infertility, defined as failure to conceive after one year of unprotected sexual intercourse. The mean exposure duration was 149 minutes per week (range 60 - 315), with a mean total duration of hyperthermic exposure of 2.9 years (range 0.75 - 10). Five subjects were exposed to hot baths, 4 to both hot baths and hot tubs, and 2 to Jacuzzi. Three of the 11 subjects had a significant smoking history (> 6 pack-years). Five others were only occasional smokers (< 1 pack-years). Complete pre-intervention semen parameters among study subjects are given in Table-2. Patients had an average of 1.6 ± 0.6 and 2.5 ± 1.7 semen analyses performed prior to cessation of exposure and following cessation, respectively. Nine men (82%) had TMC < 20 x 106 (mean 5 x 106). The other 2 men had TMC’s of 34 x 106 and 117.6 x 106 sperm.





Response to Cessation of Wet Heat Exposure
     Semen analyses for each subject were categorized into baseline (during exposure), 0 to 3 months post-intervention, and > 3 months post-intervention. Multiple semen analyses within a given period were averaged for each individual during that period. Sperm parameters before and after discontinuation of exposure are listed in Table-3. TMC values and response to intervention status are shown in Table-4. Mean follow-up of the study cohort was 7 months (range 2-16). Amongst the entire cohort, improvement in TMC did not reach statistical significance (p = 0.89). Overall, 44.5 % (95% CI 16.7,76.7) of men showed > 200% increase in total motile sperm count after discontinuation of wet heat exposure and were considered responders to intervention.





     Among responders, the mean increase in TMC was 491% (range 221-873), excluding an outlier whose TMC improved from zero to 200,000 sperm. Responders’ TMC before and after intervention are illustrated in Figure-1. The seminal response in the responding cohort showed increases in sperm concentration, but largely the result of increases in sperm motility. Although the increase in TMC increase did not reach statistical significance (p = 0.07) among responders, a subset analysis showed a mean increase of 22% in sperm motility, which was statistically significant (p = 0.02). Among responders, one patient had a TMC increase from 0 to 0.2 million sperm, two patients had post-intervention TMC’s of 9.3 and 10.7 x 106, while two more had a TMC’s of > 20 x 106 sperm. There was no significant difference in the length of follow-up among study responders and non-responders.



     Factors that could explain a response or lack of response to the intervention were examined in both cohorts. Tobacco use emerged as a possible differentiating factor. Among 6 non-responders, 5 were chronic tobacco users with a significant smoking history (mean 5.6 pack-years), compared with 3 occasional smokers in the responder group, whose mean tobacco consumption was 0.11 pack-years. The prevalence of varicoceles was similar in responders and non-responders (4/5 and 4/6 patients, respectively). No other potential gonadotoxic factors were identified in the study cohort.

COMMENTS

     The removal of wet heat exposure resulted in improvement in semen quality in nearly one-half of subjects studied. The improvement was largely the results of increases in sperm motility, and appeared to persist beyond 3 months, although a continuous improvement was not observed in all subjects. The extra-abdominal position of the scrotum and the proximity of the pampiniform venous plexus to the testicular arteries contribute to the efficient dissipation and transfer of heat away from the testes and resultant lower testicular temperature (8). However, in the presence of extreme elevation of extra-testicular temperature, as when immersed in hot liquid, these same characteristics make the scrotum particularly susceptible to deleterious thermal effect. It is therefore not surprising that semen quality might be susceptible to the effects of wet hyperthermia.
     The deleterious effect of dry heat on semen quality and, by extrapolation, male fertility, has been recognized medically for decades, and to the traditional medicine community for millennia. Carlsen et al., in examining the effect of febrile illness on semen quality, demonstrated a dose-response relationship between the number of days with fever and sperm concentration (8). Similarly, Mieusset & Bujan examined mild testicular heating (about 1°C) as a form of contraception, an idea originally conceived of by Robinson et al. (14,15). Both studies found that sperm concentration rebounded to baseline in 12 to 18 months following cessation of increased testicular temperatures. None of these studies, however, were designed to replicate the frequent, significant wet hyperthermia that is the fate of the habitual hot-tuber, thus forming the rationale for this study.
     In reviewing our data on the recovery of semen quality in infertile males following the cessation of wet heat exposure, we found that there were two distinct groups of patients: those that responded to intervention and those that did not. Among responders, improvements in semen quality were witnessed well beyond the 3 month period typically ascribed to a single cycle of spermatogenesis. This finding is entirely consistent with the time course of recovery noted after varicocele repair and exposure to other gonadotoxins (16). Interestingly, the semen analysis parameter that exhibited the largest change among responders was sperm motility, and this increase reached statistical significance after intervention. This suggests that heat-induced motility dysfunction may be the parameter most vulnerable to wet hyperthermic exposure. Although statistical significance was not achieved in the overall increase in TMC after intervention, the changes observed could be considered clinically significant, as more men could qualify for IUI instead of IVF for infertility treatment following intervention. Given that intrauterine insemination (IUI) can be considered for low semen quality in men with TMC > 8-10 x 106 sperm, the number of men who would qualify for IUI in the responder group increased from 1/5 pre-intervention to 4/5 post-intervention. Two of the responders had TMC > 20 x 106 after intervention and were advised to consider unprotected intercourse before IUI. This “shift of care” to less intensive forms of assisted reproduction has also been described for varicocele repair (16). We acknowledge that even statistically significant changes in semen parameters are always problematic to assess, given the naturally high intra-individual variability of semen parameters in both fertile and infertile men. However, the potential clinical value of these changes must also be considered, as we have outlined.
     Careful analysis of non-responders suggests that chronic tobacco use was more common among non-responders, suggesting that it may complicate the recovery from wet heat exposure. This finding is hardly surprising, considering that tobacco use is a well established, independent risk factor for poor semen quality (17-19). It is interesting to speculate whether the concomitant exposure to tobacco and wet heat may act synergistically to prevent early and rapid recovery of semen quality.
     It is interesting that two responders demonstrated subtle declines in TMC following the 3-month time interval. This lack of continuous improvement suggests that peak improvements in TMC may be time-limited and could involve other, less well-described physiological responses. It may also suggest that a timeline for therapy be offered to affected patients, to avoid prolonged follow-up without obvious benefit.
     Because this is a small study, the results should be confirmed in a larger series of patients. Given the complexity of factors underlying male infertility, including currently uncharacterized factors, more accurate results will only be generalizable with a larger study. We plan further follow-up on the patients in this study to assess conception after elimination of hyperthermia.

CONCLUSIONS

     This study addressed the relatively unsubstantiated issue of wet heat exposure as a factor in male infertility. We demonstrated that infertile men who are frequently exposed to wet heat in the form of hot tubs, Jacuzzis, or hot baths, may realize a marked increase in semen quality following cessation of exposure. The response persists for more than 3 months, and is driven mostly by the increase in sperm motility.

CONFLICT OF INTEREST

None declared.

ACKNOWLEDGEMENT

     Doctor Shai Shefi is Recipient of fellowship from The American Physicians Fellowship for Medicine in Israel.

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____________________
Accepted after revision:
October 18, 2006

_______________________
Correspondence address:
Dr. Paul J Turek
Department of Urology, UCSF
1600 Divisidero St, Rm A633
San Francisco, CA, 94143-1695, USA
E-mail: pturek@urology.ucsf.edu